Comparable brightness and greater resolution relative to FITC
High-expression intracellular markers can be resolved comparably with FITC and BD Horizon™ RB545 Reagents.
PBMCs were stimulated with PMA and ionomycin in the presence of
BD GolgiStop™ Protein Transport Inhibitor for 5 hours and then stained with either BD Horizon™ RB545 Reagents or FITC IFN-γ (B27) and matched isotype controls. Samples were acquired on a BD FACSymphony™ A5 SE Cell Analyzer.
Stable performance with lot-to-lot consistency
RB545 lot-to-lot consistency is demonstrated across specificities
Human whole blood was stained with human CD4 (SK3) RB545, followed by lysis with BD FACS™ Lysing Solution using four different batches of reagent. Mouse splenocytes were stained with mouse CD8a (53-6.7) RB545 using four different batches of reagent. All specificities were run on a BD FACSymphony™ A5 SE Cell Analyzer.
RB545 reagents can be used together with FITC to expand parameters in spectral flow cytometry
RB545 and FITC used together in a multicolor TBNK panel.
Human whole blood was stained with eight dyes and lysed with BD FACSLyse™ Lysing Buffer. Samples were then acquired on a BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed using FlowJo™ Software. RB545 and FITC BB515 and FITC Similarity Index
Using RB545 with FITC or the BD Horizon Brilliant™ Blue 515 (BB515) Reagent is a superior alternative to using FITC with BB515.
Human PBMCs were stained with two sets of fluorochromes, moderately similar RB545 and FITC, and highly similar BB515 and FITC. Co-expressed or not co-expressed markers were selected to demonstrate impact of spectral unmixing with the different levels of similarity. RB545 with FITC provides better population resolution compared to FITC with BB515. Samples were acquired on the BD FACSymphony™ A5 SE Cell Analyzer and spectrally unmixed with FlowJo™ Software.
