BD Horizon RealBlue™ 780 Reagents:
The superior alternative to PE-Cy7
BD Horizon RealBlue™ 780 reagents leverage an innovative laser-specific fluorochrome, excited primarily by the 488 nm blue laser and offer:
- Minimal cross-laser excitation of the 561-nm yellow-green laser
- Bright fluorochrome to support the detection of low-expression
surface and intracellular markers - Reduced monocyte background compared to PE-Cy7
BD Horizon RealBlue™ 780 reagents can be used with PE-Cy7 on appropriately configured conventional and spectral cytometers with both a 488-nm blue and 561-nm yellow-green laser to increase the number of experimental parameters.
Key attributes of RealBlue™ 780
Bright & clean – RealBlue™ 780 is brighter than PE-Cy7 on average. It offers minimal nonspecific binding to monocytes, low spillover due to minimal cross-laser excitation off the yellow-green laser, and enables simultaneous use of detectors on the blue and yellow-green laser lines.
Optimized for spectral – RealBlue™ 780 facilitates high-parameter experiments with cutting-edge technologies, from spectral flow cytometry to image-based cell sorting.
Versatile applications – RealBlue™ 780 supports detection of varying levels of marker expression, and can be used instead of PE-Cy7 or with PE-Cy7. Available in a wide range of specificities.
Stable performance – RealBlue™ 780 offers stable reagent performance with lot-to-lot consistency.
Broadly compatible – RealBlue™ 780 reagents are compatible with a variety of common fixation and permeabilization systems.
Uncover clonal diversity and function of full-length V(D)J sequences for B and T cells at the single cell level. Add multiomics to your research and understand clonotype information, gain insight into basic biology of these clonotypes and their disease states. We are here to help you simplify the complexity in multiomics and flowcytometry.
