Bright and Clean
- Primarily excited by the 488-nm blue laser
- Minimal cross-laser excitation off the 561-nm yellow-green laser
- Bright fluorescence to detect low-expression markers
On average, RB780 is brighter than PE-Cy7 and BB790-P.
Stain index (SI) data showing a comparison of
BD Horizon™ RB780, PE-Cy7, and BD Horizon™ BB790-P CD4 (SK3) Reagent off the 488-nm blue laser (B810) and PE-Cy7 off the 561-nm yellow-green laser (YG780) in healthy PBMC samples. SI data displayed as a percentage of RB780 SI.
RB780 is primarily excited by the 488-nm laser and shows significantly lower spillover spread into detectors on the yellow-green laser.
Versatile
- Supports the detection of a wide variety of specificities, including low-expression surface and intracellular markers
- Outperforms PE-Cy7 in brightness across multiple instruments
- Significantly less background from nonspecific monocyte binding than PE-Cy7
RB780 reagents produce lower background than PE-Cy7, enabling clear resolution of subpopulations.
Human whole blood was stained with PE-Cy7 or BD Horizon™ RB780 Human CD3 (UCHT1), CD4 (SK3), CD8 (RPA-T8), or CD19 (SJ25C1) Reagents, followed by lysis with BD FACS™ Lysing Solution. All specificities were acquired off the blue laser on a BD FACSymphony™ A5 SE Cell Analyzer.
The Q&A on BD Horizon RealBlue™ 780
Can RB780 reagents be used with PE-Cy7 in a panel on a conventional flow cytometry instrument?
Yes, if the instrument has appropriate filters for PE-Cy7 on both the blue and yellow-green lasers.
Can RB780 reagents be used together with PE-Cy7 on a BD LSRFortessaTM Cell Analyzer?
No, not in the standard configuration.
What is the size of the RB780 fluorochrome?
Less than 30 kDa.
Are RB780 reagents based on polymer technology?
RB780 reagents use a proprietary next-generation tandem dye technology that is different from Sirigen polymer technology.
Do RB780 reagents need special buffers or handling to prevent dye-to-dye interactions?
No. However, for human whole blood specimens we recommend using BD HorizonTM Brilliant Stain Buffer (BSB) to minimize possible background that may be caused by anti-PEG antibodies.
Are RB780 reagents compatible with viability dyes?
Yes, RB780 reagents are compatible with all viability dyes.
