Understand the intricacies of your cellular populations by assembling the full-length TCR and BCR sequences in combination with RNA and cell surface protein characterization at single-cell resolution
BD Rhapsody™ TCR/BCR Multiomic Assay Kit: A full-length T cell and B cell receptor profiling tool for single-cell studies
The BD Rhapsody™ TCR/BCR Multiomic Assay Kit is a full multiomics solution that enables scientists to get a comprehensive understanding of immune cell function, providing critical insight for immunology and immuno-oncology research applications. This 8-page brochure explains how you can use the BD Rhapsody™ TCR/BCR Multiomic Assay Kit to profile full-length B cell and T cell receptors in combination with cell surface protein staining and targeted or whole transcriptome mRNA analysis.
Topics include:
Study parameters and results of an analysis of TCR, BCR, whole transcriptome
gene expression and cell surface proteins
In this study, we simultaneously analyzed TCR, BCR, whole transcriptome gene expression as well as cell surface proteins in two multiplexed samples. Resting PBMCs, stained with the BD AbSeq Immune Discovery Panel (IDP) + 4 BD AbSeq Antibody drop-ins, were multiplexed with enriched T cells from a di!erent donor, stained with a 15-plex BD AbSeq Panel.
Applications of interest
The BD Rhapsody™ TCR/BCR Multiomic Assay Kit was developed for multiple application types:
- Screen for antigen-specific cells in response to infectious diseases
- Profile the immune repertoire of tumor infiltrating lymphocytes
- Vaccine research
- Understand transplant rejection or tolerance
- Discover biomarkers for immunotherapy
- Pinpoint and characterize new approaches for cell therapies
Benefits of the BD Rhapsody Assay Kit
The BD Rhapsody™ TCR/BCR Multiomic Assay Kit is part of the broad portfolio of reagents, instruments and software designed to support your single-cell research. Easily design your experiments with validated protocols and products and find everything you need from your trusted partner.
The advantages include:
full-length assay
3′ gene expression
detection of rare cell types
sample multiplexiing
savings on sequencing costs
Uncover clonal diversity and function of full-length V(D)J sequences for B and T cells at the single cell level. Add multiomics to your research and understand clonotype information, gain insight into basic biology of these clonotypes and their disease states.
We are here to help you simplify the complexity in multiomics and flowcytometry.