Introducing BD Horizon RealYellow™ 586 Reagents:

engineered to help you spend less time optimizing panels and more time discovering

Request a discount code for RealYellow™ 586

Bright and Clean

Bright fluorochrome to support the detection of markers with varying levels of expression.
Minimal background.
Minimal cross-laser excitation off the blue, violet and UV lasers.
Less spillover spread into multiple blue channels and select violet and UV channels
compared to PE

RY586 is brighter than PE and other fluorochromes.

Mean fluorescence intensity and stain index data show that RY586 CD4 is brighter than PE, Spark YGTM 581 and NovaFluorTM Yellow 570 CD4 reagents. Similar trends were seen for other specificities.

RY586 exhibits significantly less cross-laser excitation by the blue, violet and UV lasers, resulting in less spillover.

This chart compares the normalized emission profile of PE and RY586, showing lower emission of RY586 into blue, violet and UV detectors.

Versatile

Supports detection of surface and intracellular markers.
Can be used instead of PE on conventional flow cytometers or with PE on spectral flow cytometers.
Available in a wide range of specificities to support a variety of applications.

Low-expression surface markers can be resolved comparably with PE and RY586.

Human whole blood was stained with RY586 (left) or PE (right, acquired off the yellow-green laser) CD25 (2A3) and acquired on a BD FACSymphony™ A5 SE Cell Analyzer.

Like PE, RY586 delivers clear resolution of intracellular markers.

Human PBMCs were stimulated with PMA and ionomycin in the presence of BD GolgiStop™ Protein Transport Inhibitor for 5 hours and then stained with APC CD4 and either RY586 (left, 0.125 μg) or PE (right, 0.25 μg, acquired off the yellow-green laser) IL-17a (N49-653) and acquired on a BD LSRFortessaTM Cell Analyzer.

Specially designed to enable high-parameter panel design for spectral flow cytometry.

The Q&A on BD Horizon RealYellow™ 586

Can RY586 be used with the 532-nm green laser instrument configuration?
No. For optimal results, please use a yellow laser (561 nm) configuration. For the 532-nm green laser configuration, we recommend using PE.

Can RY586 be used with PE in a panel on a conventional flow cytometry instrument?
Yes, if the instrument has PE-appropriate filters on both the blue and yellow-green lasers.

Is RY586 based on polymer technology?
No, RY586 is a specially engineered small molecule organic fluorochrome.